Biologi Bm Regenerate Anti-Ageing Serum
Biologi Bm Regenerate Anti-Ageing Serum
Bm Regenerate Anti-Ageing Serum is Biologi’s single-plant ingredient serum setting new standards in anti-ageing skincare. Featuring the hero ingredient Australian grown Mountain Pepper Berry, this serum enhances collagen production, repairs barrier function and increases hydration to gently defy ageing signs.
Studies have shown that using microbiome-supporting skin care gives improved results in both the skin microbiome and biophysical parameters compared to conventional skin care products. The peptide complex with stimulatory activities on the epidermal proteins collagen XVII, laminin and nidogen was developed.
Anti-wrinkle activity
The basement membrane (BM) is a complex extracellular matrix that provides cell-adherent structures, connects the epidermis to the dermis and supports molecular transport between them. It contains structural proteins such as laminins, collagen IV and nidogens. The reduction in expression of these BM proteins is associated with wrinkle formation [1].
BM skin care products can support the synthesis of these proteins by activating epidermal keratinocytes. This can result in a reduction of wrinkles, a firmer and more resilient appearance of the skin, and improved skin elasticity.
For example, a serum containing Tasmannia lanceolata extract has been shown to stimulate the synthesis of the BM protein collagen XVII, a key regulator of fibroblast activity and the formation of new collagen fibers. In addition, it has been shown to inhibit the degradation of existing collagen strands and to promote their reorganization into more mature fibers.
In another study, researchers found that peptides from the BM-associated protein -Syntaxin 1 showed anti-wrinkle effects by enhancing epidermal keratinocyte BM skin care proliferation and stimulating collagen synthesis in vivo and ex vivo. This effect was further enhanced by the use of biotinylated peptides and Vitamin C conjugated peptides. This combination triggered an increase in the expression of the BM proteins laminin IV and nidogen, as well as an improvement in wrinkles.
Stimulation of BM proteins
In skin BMs are crucial for epidermal barrier functions protecting against environmental impacts and microbes. They link the epidermis to the extracellular matrix of the dermis through a tight bond between the anchoring fibrils (hemidesmosomes) consisting of intracellular plaque proteins plectin and bullous pemphigoid antigen 1 and 2, and laminin g1 chains which have nidogen binding sites. Nidogen-1 deficiency or ablation results in a loss of hemidesmosomes and a general disassociation of BM components but does not affect perlecan binding to laminin g1 chains [105]. In mice lacking both nidogens BMs are still formed though they have specific defects in the lungs, heart, nerves, and skeletal muscle. Moreover, nidogen-2 redistributes to BM components and compensates for nidogen-1 in most organs and tissues including the skin [106].
In 3D cocultures fibroblasts with a nidogen deficiency showed impaired BM formation as monitored by immunofluorescence. Compared to controls, nidogens no longer decorated the cells and laminin g1 chain laminins were lost with some delay followed by collagen IV and a reduced amount of integrin b1 tetraspanin. Nevertheless, a continuous staining of the cell-matrix interface could still be observed. The fact that nidogens act also in other functions than their BM-building function suggests that they might be involved in signaling processes. This would be supported by the high susceptibility of nidogens to proteases like matrix metalloproteases or cathepsin S.
Biotinylated peptides
The most common method for labeling proteins is to use biotin. However, this technique has a number of disadvantages. The label is often introduced at the N-terminal end of a protein, which can interfere with protein function. Additionally, N-terminal biotinylation may also have poor solubility. Biotinylation at the C-terminus is often more effective. This technique is used to label proteins that can then be isolated and studied for their function.
This can be done by using a streptavidin-biotin binding pair. Streptavidin and avidin have a tetrameric structure, which allows them to bind up to four different ligands. This enables researchers to determine the binding partners of a particular protein.
To use a peptide as a bait, it is first immobilized onto streptavidin-coated beads. Then, it is incubated with your target sample, such as a cell lysate or purified protein. The resulting bound proteins can then be eluted and analyzed by SDS-PAGE. By comparing the resulting protein binding data with controls, you can identify the specific function of your target protein.
To make biotinylated peptides, you can use a variety of biotin-labelling reagents. Most commonly, the peptide is biotinylated by an enzyme, such as the biocytin-biotin ligase in vivo. The resulting biotin-peptide complex is then bound to streptavidin-coated beads. These beads are then incubated with your target sample, such a cell lysate or purified recombinant protein.
Vitamin C conjugated peptides
A vitamin C-conjugated peptide is an ester of the peptide Glycerinated Ethylglucose Carboxylate (GEKG) and vitamin C. This molecule has been shown to stimulate collagen synthesis and related gene expression more effectively than vitamin C or GEKG alone. It is also effective in improving skin color. It acts as a natural skin lightener and is effective without irritating the skin.
Melanin, a pigmented molecule, is synthesized by the melanocyte in BM skin care shop the basal layer of the epidermis and transferred to peripheral keratinocytes to determine human skin color. Melanin is a free-radical inhibitor and protects the skin from oxidative damage. It is also effective in the treatment of inflammatory acne.
BM skin care products combine the regenerating effect of glycolic acid with the skin brightening power of ubiquinone. This powerful combination promotes skin regeneration and protects the skin from environmental damage. The products also reduce blemishes and redness to improve the skin tone. In addition, they also have antioxidant properties. These products are ideal for daily use. They can be used as cleansers, toners or moisturisers.